Application of neighborhood-scale wastewater-based epidemiology in low COVID-19 incidence situationsArticle Published on 2022-12-152022-11-16 Journal: The Science of the total environment [Category] COVID19(2023년), SARS, 변종, [키워드] application applied approach average characterized Concentration COVID-19 COVID-19 incidence Disease surveillance effective Epidemiology incidence rate infections less limit local outbreak Low COVID-19 incidence Mild N gene Neighborhood-scale sewersheds normalized outbreak plant public health measure quantification reported RT-qPCR SARS-CoV-2 SARS-CoV-2 N gene SARS-CoV-2 variant-specific RT-qPCR assays sensitivity Seven specificity statistically Surveillance the SARS-CoV-2 threshold Treatment virus Wastewater-based epidemiology. [DOI] 10.1016/j.scitotenv.2022.158448 PMC 바로가기
Integration of RT-LAMP and Microfluidic Technology for Detection of SARS-CoV-2 in Wastewater as an Advanced Point-of-Care PlatformArticle Published on 2022-12-012023-07-09 Journal: Food and Environmental Virology [Category] COVID19(2023년), [키워드] Microfluidic device N gene RT-LAMP SARS-CoV-2. [DOI] 10.1007/s12560-022-09522-3 PMC 바로가기
RT-LAMP assay for rapid detection of the R203M mutation in SARS-CoV-2 Delta variantSARS-CoV-2 Delta 변이체에서 R203M 돌연변이의 신속한 검출을 위한 RT-LAMP 분석Article Published on 2022-12-012022-09-11 Journal: Emerging Microbes & Infections [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] Accuracy amplification analyses appear AUC clinical samples conserved COVID-19 COVID-19 vaccines Delta delta variant Delta variants detect determined by Diagnosis discrepancy dominant Efficiency facilitate genotyping infected individuals Mutation N gene positive sample Primers quantification quarantine R203M mutation Rapid rapid diagnosis. RT-LAMP RT-qPCR SARS-CoV-2 sensitivity sequence Sequencing sets of primer sets of primers specificity specimen Surveillance threshold variant variants [DOI] 10.1080/22221751.2022.2054368 PMC 바로가기 [Article Type] Article
Dumbbell-type triplex molecular switch-based logic molecular assays of SARS-CoV-2Article Published on 2022-11-152022-10-06 Journal: Sens Actuators B Chem [Category] 진단, [키워드] acute respiratory syndrome amplification approach caused coronavirus COVID-19 COVID-19 pandemic cross-contamination Detection limit diagnostics Dumbbell-type triplex molecular switch Epidemics Frame gold standard High-fidelity independent Logic-gate molecular Molecular assay N gene nucleic acid amplification ORF1ab probe recognizing Region RT-PCR SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 RNA. spacer [DOI] 10.1016/j.snb.2022.132579 PMC 바로가기 [Article Type] Article
Clinical predictors and RT-PCR profile of prolonged viral shedding in patients with SARS-CoV-2 Omicron variant in Shanghai: A retrospective observational studyArticle Published on 2022-10-242022-11-16 Journal: Frontiers in Public Health [Category] COVID19(2023년), SARS, 변종, [키워드] acute respiratory syndrome Admission analysis Analysis clearance clinical Comorbidity Coronary heart disease coronavirus Ct values cycle threshold determinant diabetes equation evaluate Frame Generalized group hospitalized patient hypertension Infection male material median age multivariable analysis N gene nasopharyngeal nucleocapsid protein omicron ORF ORF 1ab gene Patient patients with SARS-CoV-2 positive predictor predictors prolonged SARS-CoV-2 RNA shedding. prolonged viral shedding proportion receiving remained retrospective Retrospective study RT-PCR SARS-CoV-2 SARS-CoV-2 Omicron variant SARS-CoV-2 RNA significantly single-center tested the timing vaccination Vaccinations Vaccine variant viral clearance viral RNA clearance Viral RNA shedding [DOI] 10.3389/fpubh.2022.1015811 PMC 바로가기
Diagnostic efficiency of RT-LAMP integrated CRISPR-Cas technique for COVID-19: A systematic review and meta-analysisArticle Published on 2022-10-012022-11-15 Journal: Pathogens and global health [Category] COVID19(2023년), SARS, 치료기술, 치료제, [키워드] Accuracy applicability article calculated Care chain conducted confidence interval COVID-19 COVID-19 diagnosis COVID-19 pandemic CRISPR-Ca CRISPR-Cas database Diagnosis diagnostic Diagnostic method Efficiency eligibility criteria epidemiological equipment False-positive faster feasible forest plot isothermal amplification limit management Meta-analysis Meta-analysis. molecular N gene Precision problem QUADAS-2 Quantitative reaction Region regions resource reverse transcription RT-LAMP RT-qPCR SARS-CoV-2 sensitivity specificity systematic review Transcription USA was performed with COVID-19 [DOI] 10.1080/20477724.2022.2035625 PMC 바로가기
RT-LAMP assay combining multi-fluorescent probes for SARS-CoV-2 RNA detection and variant differentiationSARS-CoV-2 RNA 검출 및 변이체 분화를 위한 다중 형광 프로브를 결합한 RT-LAMP 분석Article Published on 2022-10-012022-09-11 Journal: Talanta [Category] COVID19(2023년), SARS, 변종, 치료기술, 치료제, [키워드] addition applied can be used clinical setting clinical settings detect Fluorescent probes Genes isothermal amplification limit of detection Loop-mediated isothermal amplification molecular mutant Mutation N gene observation ORF1 ORF1ab P681H P681R positive result primer set probe quantification regions reverse transcription Reverse transcription loop-mediated isothermal amplification (RT-LAMP) RT-LAMP S gene SARS-CoV-2 SARS-CoV-2 RNA SARS-CoV-2 RNA detection SARS-CoV-2 viral RNA sensitivity simple specificity Spread Transcription type strain variant Variant differentiation. viral RNA detection virus wild type wild type strain wild-type [DOI] 10.1016/j.talanta.2022.123644 PMC 바로가기 [Article Type] Article
Nasopharyngeal Wash with Normal Saline Decreases SARS-CoV-2 Viral Load: A Randomized Pilot Controlled TrialArticle Published on 2022-09-272022-11-16 Journal: Canadian Respiratory Journal [Category] SARS, 변종, [키워드] 24 hour acute respiratory syndrome administration Admission Antigen baseline control group Controlled Controlled trial coronavirus COVID-19 disease cycle threshold decrease demonstrated determine Epidemiology Fatality rate Follow-up healthcare Hospitalization hospitalized patient increase in microbial morbidity N gene nasal washe nasopharyngeal Nasopharyngeal swab nasopharyngeal viral load non-COVID-19 patient normal outcome Pathogenesis Patient performed Pneumonia previous study Randomized reduce reduction in resource Saline SARS-CoV-2 significant decrease significantly the SARS-CoV-2 Treatment two group two groups variant Viral load Viral transmission was collected was performed with COVID-19 [DOI] 10.1155/2022/8794127 PMC 바로가기
Platinum nanoparticles (PtNPs)-based CRISPR/Cas12a platform for detection of nucleic acid and protein in clinical samplesArticle Published on 2022-09-082022-11-15 Journal: Analytica chimica acta [Category] SARS, 치료기술, [키워드] acute respiratory syndrome Antigen Biomarker Biomarkers biosensor clinical sample Concentration contagious coronavirus CRISPR/Cas12a detect Detection sensitivity diagnostic Diseases identify N gene Noble metal nanocatalysts nucleic acid Nucleic acid detection optical platform Platinum Protein Protein detection Quantitative reduction reported Research RT-qPCR SARS-CoV-2 SARS-CoV-2. sensitivity specificity specimens [DOI] 10.1016/j.aca.2022.340203 PMC 바로가기
Performance evaluation of antigen detection rapid diagnostic test (Ag-RDT) for COVID-19 diagnosis in a primary healthcare center during the Shanghai COVID-19 quarantine periodArticle Published on 2022-09-012022-11-15 Journal: Virology Journal [Category] SARS, 치료기술, [키워드] Ag-RDT Antigen antigen detection Antigen rapid diagnostic test (Ag-RDT) Asymptomatic Asymptomatic COVID-19 average conducted Coronavirus disease 2019 COVID-19 COVID-19 diagnosis cycle threshold Department diagnosis of SARS-CoV-2 Emergency enrolled evaluate healthcare Inconclusive individual Infection N gene ORF1ab ORF1ab gene Patient care performance quarantine Rapid Rapid diagnostic test reliability RT-PCR SARS-CoV-2 SARS-COV-2 infection screened sensitivity Sensitivity. Shanghai significantly higher specificity was performed [DOI] 10.1186/s12985-022-01871-6 PMC 바로가기